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Our laboratory shown that SERT and αvβthree receptors colocalize in midbrain presynaptic terminals (Mazalouskas et al., 2015), suggesting that SERT is localized to multiprotein complexes coordinated by integrin αvβ3 in midbrain synapses. Prior experiments recommend that in platelets, the Affiliation of SERT with activated αIIbβthree integrins, and its localization to adhesion complexes, are correlated with improved uptake action (Carneiro et al.
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Mice had been put on an elevated round observe apparatus ∼65 cm in diameter. Two open zones and two shut zones, of equal size and all evenly spaced, have been contained about the circular monitor. One particular mouse was placed in the course of an open zone and permitted to investigate the maze for five min.
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Movie of your mouse was received and analyzed utilizing ANY-maze software (Stoelting) for your amount of time invested in Each individual zone, the volume of entries into Each and every zone, and also the distances traveled. Entry of a zone was digitally quantified based on the criterion of ≥70% of the human body in the animal remaining in a very zone.
), suggesting that constitutive activation of integrin βthree decreases the association of SERT with integrin adhesion complexes.
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